plasmid containing chtog cdna Search Results


chtop overexpression vector  (Addgene inc)
90
Addgene inc chtop overexpression vector
Chtop Overexpression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+containing+chtog+cdna/pmc08128508-85-0-8?v=Addgene+inc
Average 90 stars, based on 1 article reviews
chtop overexpression vector - by Bioz Stars, 2026-07
90/100 stars
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chtog cdna  (Addgene inc)
91
Addgene inc chtog cdna
Figure 1. CLASP1 promotes the depolymerization of GMPCPP-stabilized microtubules in a GTP-dependent manner. (A) Schematic of the microtu- bule depolymerization assay. (B) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM <t>chTOG,</t> or 200 nM CLASP1. (C) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1 in the presence of 1 mM GTP. (D) Quantification of microtubule depolymerization rates for the conditions in B and C. N = 50–108 microtubules for each condition across at least two experimental days. Individual data points from different experiments are plotted in different shades, and the means for each experimental repeat are plotted as larger points in the same color. The squares indicate the average of the experimental means, and the vertical bars are the standard errors of the means. See also Video 1. For comparison between the no GTP conditions, a one-way ANOVA followed by Tu- key’s HSD test for multiple comparisons found that the mean rate of microtubule depolymerization was significantly different between the buffer control vs. chTOG (P < 0.001) and chTOG vs. CLASP1 (P < 0.001) in the absence of GTP. There was no statistically significant difference between the buffer control and CLASP1 condition (P = 0.06) in the absence of GTP. Welch’s two-tailed unequal variances t tests were performed for pairwise comparison of the no-GTP vs. GTP conditions and the corresponding P values are indicated on the graph.
Chtog Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+containing+chtog+cdna/pm37184584-253-3-13?v=Addgene+inc
Average 91 stars, based on 1 article reviews
chtog cdna - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
chtop mouse shrna plasmid  (OriGene)
N/A
Chtop Mouse 4 unique 29mer shRNA constructs in lentiviral GFP vector
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chtop lentiviral activation particles  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of CHTOP-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
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chtop shrna (h) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CHTOP gene silencing results, individual duplex components or plasmids are also available upon request.
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c1orf77 chtop human shrna plasmid kit  (OriGene)
N/A
CHTOP Human 4 unique 29mer shRNA constructs in lentiviral GFP vector
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chtop crispr/cas9 ko plasmid  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of CHTOP-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier
chtop (m)-pr  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CHTOP gene silencing results, individual duplex components or plasmids are also available upon request.
   Buy from Supplier
chtop (h)-pr  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CHTOP gene silencing results, individual duplex components or plasmids are also available upon request.
   Buy from Supplier
chtop sirna  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CHTOP gene silencing results, individual duplex components or plasmids are also available upon request.
   Buy from Supplier
chtop shrna (m) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CHTOP gene silencing results, individual duplex components or plasmids are also available upon request.
   Buy from Supplier
chtop hdr plasmid  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of CHTOP-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier

Image Search Results


Figure 1. CLASP1 promotes the depolymerization of GMPCPP-stabilized microtubules in a GTP-dependent manner. (A) Schematic of the microtu- bule depolymerization assay. (B) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1. (C) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1 in the presence of 1 mM GTP. (D) Quantification of microtubule depolymerization rates for the conditions in B and C. N = 50–108 microtubules for each condition across at least two experimental days. Individual data points from different experiments are plotted in different shades, and the means for each experimental repeat are plotted as larger points in the same color. The squares indicate the average of the experimental means, and the vertical bars are the standard errors of the means. See also Video 1. For comparison between the no GTP conditions, a one-way ANOVA followed by Tu- key’s HSD test for multiple comparisons found that the mean rate of microtubule depolymerization was significantly different between the buffer control vs. chTOG (P < 0.001) and chTOG vs. CLASP1 (P < 0.001) in the absence of GTP. There was no statistically significant difference between the buffer control and CLASP1 condition (P = 0.06) in the absence of GTP. Welch’s two-tailed unequal variances t tests were performed for pairwise comparison of the no-GTP vs. GTP conditions and the corresponding P values are indicated on the graph.

Journal: The Journal of cell biology

Article Title: CLASPs stabilize the pre-catastrophe intermediate state between microtubule growth and shrinkage.

doi: 10.1083/jcb.202107027

Figure Lengend Snippet: Figure 1. CLASP1 promotes the depolymerization of GMPCPP-stabilized microtubules in a GTP-dependent manner. (A) Schematic of the microtu- bule depolymerization assay. (B) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1. (C) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1 in the presence of 1 mM GTP. (D) Quantification of microtubule depolymerization rates for the conditions in B and C. N = 50–108 microtubules for each condition across at least two experimental days. Individual data points from different experiments are plotted in different shades, and the means for each experimental repeat are plotted as larger points in the same color. The squares indicate the average of the experimental means, and the vertical bars are the standard errors of the means. See also Video 1. For comparison between the no GTP conditions, a one-way ANOVA followed by Tu- key’s HSD test for multiple comparisons found that the mean rate of microtubule depolymerization was significantly different between the buffer control vs. chTOG (P < 0.001) and chTOG vs. CLASP1 (P < 0.001) in the absence of GTP. There was no statistically significant difference between the buffer control and CLASP1 condition (P = 0.06) in the absence of GTP. Welch’s two-tailed unequal variances t tests were performed for pairwise comparison of the no-GTP vs. GTP conditions and the corresponding P values are indicated on the graph.

Article Snippet: The plasmid containing chTOG cDNA was a gift from Stephen Royle (plasmid #69108; Addgene; http://n2t.net/addgene:69108; RRID: Addgene_69108).

Techniques: Incubation, Comparison, Control, Two Tailed Test